Antimicrobial activity of methanolic extract of flowers of. Plant extracts were obtained with a methanol or methanolwater 1. The screening of plant extracts and plant products for antimicrobial activity has shown that plants represent a potential source of novel antibiotic prototypes riaz et. The content of total phenolics in the extracts was determined spectrometrically according to the folin. Plant extract of 1, 10, 50 or 100 mg in 1 ml of ethanol was mixed with 2.
The results clearly show that the plant extracts were specific in action against the growth of bacteria. The three flavnoids present in the crude extract and enriched in flavnoid fraction are rutin, aspigenin7oglucoside and luteolin7oglucoside. All the extracts inhibited mycellial growth at various levels. Phytochemical investigation and biological activity of. Cytotoxicity and antibacterial activity of methanolic extract of hibiscus sabdariffa olaleye, mary tolulope department of biochemistry, federal university of technology, akure, nigeria. Present study was undertaken to determine phytochemical content and ros scavenging inhibitory activity of 11 medicinal plants from western ghats of india in order to evaluate their potential as a natural antioxidative source. Antioxidant activity of plant extracts and their effect on. The effect of various plant extracts was measured on several important oxidation indicators. Cytotoxicity and antimicrobial activity of selected plant species.
This is a good study in which the authors evaluated the antioxidant activity of extracts of root, stem, leaf, flower and fruit of l. The full text of this article is available in pdf format. Antifungal activity of selected medicinal plant extract on. The plant extracts showed a considerable range of influence on cytokine secretion. The antifungal activity of ethanol and acetone extract of leaves of nine medicinal plants. Antioxidant activity of plant extracts containing phenolic. Effect of solvent on antioxidant activity of crude. Antibacterial activity of crude extracts of some south african. In vitro antioxidant and anticancer activity of leea. Aqueous, saline buffer and acid extracts of different plant species were screened in vitro for their antifungal activity against alternaria spp. Invitro screening of acetylcholinesterase inhibitory. It enables identification of the intrinsic toxicity of the plant and the effects of acute overdose.
The antioxidative activity of a total of 92 phenolic extracts from edible and nonedible plant materials berries, fruits, vegetables, herbs, cereals, tree materials, plant sprouts, and seeds was examined by autoxidation of methyl linoleate. Plant oils contain unsaturated fatty acids, which are most sensitive to oxidative degradation. The study of the antioxidant activity of oil extracts from. The aims of this study were to utilize some crude plant extracts in developing yogurt of high acceptability. The study of the antioxidant activity of oil extracts from the seeds of two sorts of red onion. The plant extracts with the most significant antiinflammatory activity are shown in fig. Evaluation of in vitro anthelmintic activity of cymbopogon citratus lemon grass extract sikandar khan sherwani 1, muhammad mumtaz khan 2, muhammad uzair khan 3, muhammad ajmal shah 4 and shahana urooj kazmi 5 1, department of microbiology, federal urdu university of arts, science and technology, karachi, pakistan. The lethal dose of the methanolic extract of the plant leaves is more than 2000mgkg. Bioguided fractionation and purification of natural. Currently, there is a growing interest to use plant extracts of herb and spices for the preservation of foods, flavor characteristic.
In our study, the reducing activity increased with respect to the concentration of different organic extract dichloromethane extract, ethyl acetate and methanol of k. Antimicrobial activity of the leaf extracts was tested using agar well diffusion method. In vitro activity of a combination of bacteriophages and. You may choose to submit your manuscript as a single word or pdf file to be used in the. Cytotoxicity and antibacterial activity of methanolic. Phytochemical analysis, antimicrobial and antioxidant. Therefore, plant oils are widely used as a substrate for testing antioxidant activity. Effects on blood glucose levels dried ethanolic extracts of barleria cristata seeds 200 mgkg were suspended in 1% bentonite and subjected for hypoglycemic activity in wistar rats 200225 g. The minimal inhibitory concentration mic values of extract against the tested bacteria were found to 67. Antioxidant activity, total phenolic and total flavonoid. Further, to investigate and estimate total phenolic content using rphplc. Phytochemical analysis and assessment of antioxidant and.
Pp fraction showed better activity than fr fraction. The order of dpph scavenging against cassia fistula extract was found to be in the order of. Free radical scavenging and total antioxidant capacity of root extracts of. These results show that the aqueous extracts of five native plants of the. Plant extracts with phytochemicals of good antimicrobial properties are of great significance in treatment of infectious diseases. Bioactive aqueous extracts of twelve yucatecan plants. Antiinflammatory activity of extracts from fruits, herbs. Evaluation of in vitro anthelmintic activity of citratus. Antioxidant, antihypertensive, antihyperglycemic, and. The revised version of the report includes substantially more information on the. In summary, this study confirms that many essential oils and plant extracts possess in vitro antibacterial and antifungal activity. Plants extracts were screened for antibacterial activity using serial. Screening of in vitro antioxidant activity of methanolic. The evaluation of the anticancer activity of plant extracts is essential for safe treatment.
Through the literature survey, the mangrove plant acanthus ilicifolius was. Staphylococcus aureus by using discdiffusion assay. All the animals were regularly observed for their general behaviour. Bacillus subtilis was partially inhibited by 15 plant extracts and weakly inhibited by the others. Medicinal plants and plant extracts scanned pdf, 2 mb author. Antioxidant activity of polyphenolic plant extracts mdpi. Nonflavonoids include stilbene, phenolic acids, saponin, and tannins 1. Tonpay1 1department of pharmacology, 2department of microbiology, gajara raja medical college, gwalior, m.
Water extract with melanin inhibition activity 266. Antioxidant activity of ginger extract and identification. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Extracts of annona species are potential antimosquito. The antioxidant activity was investigated using the ferric reducing antioxidant power frap assay for plant extracts was investigated according to the method of oyaizu 1986 27. The effect of some medicinal plant extracts on biochemical. The medicinal plant extracts exhibited stronger antidermatophytic activity compared to the algae extracts. Concentrationresponse curves for the antiproliferative. Antioxidant activity of the methanol extracts of pulp and seed was also compared to total phenolic content and it was further found that radical scavenging effects of extracts were directly proportional to the phenolic content present in extracts table 1.
Antibacterial activity of plant extracts and phytochemicals on. Secretion of the proinflammatory cytokine, il6, was significantly reduced by at least 25. Antioxidant, antimicrobial activity and total phenolic. However, if plant oils and extracts are to be used for food preservation or medicinal purposes, issues of safety and toxicity will need to be addressed. Identification of targets and mechanism of activity of plant extracts 5. Assessment of antioxidant activity of plant extracts by. Estimation of phytochemical content and antioxidant. As a rule they are obtained by maceration extraction to equilibrium with water or alcohol or percolation extraction to exhaustion with water or alcohol. This is a pdf file of an unedited manuscript that has been accepted for. The total phenolic contents tpc and the antioxidant activities of water and ethanol extracts of 14. Association of antibiotics and plant extracts showed synergistic antibacterial activity against antibioticresistant bacteria. Guide for authors fitoterapia issn 0367326x elsevier.
Page 9 g and an excellent source of vitamin c ascorbic acid. To test the antimicrobial activity, all plant extracts were dissolved in pure dimethyl sulfoxide dmso to a final concentration of 100 gl 100 mg plant extract in 1 ml of dmso. Saroj kothari1, vaibhav mishra2 savita bharat2 and shrinivas d. Antioxidant activity of plant extracts and their effect on methane fermentation in bioreactors marta oleszek1 and solomiia kozachok2,3 1institute of agrophysics, polish academy of sciences, doswiadczalna 4, 20290 lublin, poland 2institute of soil sciences and plant cultivation, state research institute, czartoryskich 8, 24100 pulawy, poland 3i horbachevsky ternopil state medical. The synthesis of metal nanoparticles using plant extracts has developed a rapid, costeffective biosynthetic protocol. Plant extracts as potential mosquito larvicides pdf. Antimicrobial and antioxidant activities of kalanchoe.
This study aims to assess the antioxidant and antimicrobial activity of the plant leave extracts of otostegia integrifolia. Antioxidant activity and the total phenolic content. Antioxidant and antimicrobial properties of five medicinal. Plasmodium spp, the vector mosquito has to be controlled. There is an increasing trend to replace synthetic antioxidants, which are of safety concern 1,2, with the natural antioxidants available from plant extracts or isolated products of plant origin. The results indicated that all tested extracts showed antioxidant activities. Among the important biological properties exhibited by plant. Present study was undertaken to determine phytochemical content and ros scavenging inhibitory activity of 11 medicinal plants from western ghats of. The highest numbers of antiradical activity units eau 515 were found for ethyl acetate extracts from the leaves of green and black tea. The antioxidative activities of six plant extracts catnip, hyssop, lemon balm, oregano, sage and thyme were evaluated in sunflower oil and its 20% oilinwater emulsion in the dark at 60c. An isolated fraction of ethanolic extracts of dregeavolubilis. Piper betel, lowsonia inermis, psidium guajava, carica papaya, moringa oleifera, mimosa pudica, catharanthus roseus, adhatoda vasica and andrographis paniculata against fusarium oxysporum the causal agent of fusarium wilt in tomato was assessed. About 50g of coarsely powdered plant materials 50g250ml were extracted in a soxhlet extractor for 8 to 10 hours, sequentially with petroleum ether. The aim of this study was to screen various solvent extracts of whole plant of torilis leptophylla to display potent antioxidant activity in vitro and in vivo, total phenolic and flavonoid contents in order to find possible sources for future novel antioxidants in food and pharmaceutical formulations.
Preparation of plant extracts fresh plant material was washed under running tap water, air dried and powdered. Several herbal plants have been used as therapeutics in iraqi traditional medicine and phenolic content and antioxidant activity may contribute to their pharmacological effects. Indeed vegetable oils contain a sufficient amount of beneficial antioxidant for man 7 can prevent many types of cancer, inflammatory and cardiovascular disease. Antioxidant activity of methanol extracts of different. Bioguided fractionation and purification of natural bioactives obtained from. Seventyfour ethanolic extracts were prepared from traditionally used medicinal plants in the jongilanga community. The flower plant extracts demonstrated good antimicrobial activity against these bacteria tested with inhibition zones. Medicinal plants and plant extracts scanned pdf, 2 mb. Activity of antibiotics plus the plant extractfractionpurified compound. Libyan capparis spinose has good antioxidant activity. The antioxidant activity of artemisia absinthum, artemisia santonicum and saponaria officinalis are given in figure 1. Plant extracts are defined as concentrated preparations of liquid, solid or viscous consistency. Antioxidant activity of plant extracts containing phenolic compounds.
Antibacterial activity of ginger zingiber officinale. Another objective of this study was to evaluate the effect of crude plant extracts additives on physical, chemical, and microbiological properties of crude. Comparative analysis of the antioxidant activity of cassia. Antimicrobial activity of essential oils and other plant. Medicinal plant extracts with efflux inhibitory activity against. Screening for antidiabetic activity of the ethanolic. Antibacterial activity of plant extracts and phytochemicals on antibioticresistant bacteria article pdf available in brazilian journal of microbiology 314 october 2000 with 1,652 reads. A 100 mgml stock of each plant extract was prepared and used for antimicrobial tests, antioxidant activity, quantification of the total phenol and total flavonoids. The ethanol extract showed the maximum activity against staphylococcus aureus and klebsiella pneumoniae with. In vitro antidermatophytic activity and cytotoxicity of extracts derived. There are statistical differences among the bha, bht and plant extracts in terms of antioxidant activity.
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